Abstract The IVF culture system is constantly being examined for means of modification to further improve conditions for gametes and embryos. Exhaustive research into physiological requirements and responses of these…
Abstract Since the birth of Louise Brown, the first ‘test tube baby’, in 1978, assisted reproductive technology (ART) has become one of the standard treatments for couples with subfertility problems….
Abstract The IVF laboratory is central to Medically Assisted Reproduction perhaps representing also the most crucial extrinsic factor in determining success or failure of treatment. Current science and technology are…
Abstract Optimal culture conditions and reliable embryo selection are two fundamental laboratory aspects of successful IVF treatment, with the overall aim being to obtain a healthy singleton pregnancy in the…
Abstract Following the fusion of the oocyte and spermatozoon and successful oocyte activation, preimplantation development begins with the formation of the zygote, displaying two pronuclei and two polar bodies if…
Abstract Incubators represent the most important piece of equipment in an in vitro fertilization (IVF) laboratory since embryos spend the largest part of their in vitro development within an incubator’s…
Abstract The embryo is a dynamic structure that can be affected by the interaction with the surrounding environment. During its journey through the female reproductive tract from fertilization to implantation,…
Abstract The purpose of this chapter is to review some of the factors that influence laboratory and clinical outcomes, broadly under the heading of optimal handling techniques. Two principal environments…
Abstract The most important purpose of embryo culture in the IVF lab is to make sure that the embryo can develop to its full potential in a safe and secure…
Abstract Before considering the basis of embryo culture medium, it is worthwhile reflecting on its function. A detailed overview of embryo metabolism is given in Chapter 4; however, in brief,…
