Chapter 2 – Incubators for Embryo Culture
Abstract Incubators represent the most important piece of equipment in an in vitro fertilization (IVF) laboratory since embryos spend the largest part of their in vitro development within an incubator’s…
GYNECOLOGY
Chapter 4 – Embryo Metabolism and What Does the Embryo Need?
Abstract The embryo is a dynamic structure that can be affected by the interaction with the surrounding environment. During its journey through the female reproductive tract from fertilization to implantation,…
Chapter 6 – Optimal Handling Techniques for Culture of Human Embryos
Abstract The purpose of this chapter is to review some of the factors that influence laboratory and clinical outcomes, broadly under the heading of optimal handling techniques. Two principal environments…
Chapter 7 – From Identification to Witnessing
Abstract The most important purpose of embryo culture in the IVF lab is to make sure that the embryo can develop to its full potential in a safe and secure…
Chapter 5 – Culture Media and Embryo Culture
Abstract Before considering the basis of embryo culture medium, it is worthwhile reflecting on its function. A detailed overview of embryo metabolism is given in Chapter 4; however, in brief,…
Chapter 3 – Consumables for the IVF Laboratory
Abstract Since the birth of Louise Brown in 1978, IVF has evolved from a research-based environment to an established and regulated clinical treatment with around 2 million treatment cycles performed…
Chapter 1 – Facilities for Embryo Culture
Abstract Cell culture laboratories in general require facilities with very high standards of hygiene and air quality. Maintaining a Grade-A environment (ISO 4 or above) in these laboratories is usually…
Chapter 36 – Allotransplantation of Human Ovarian Tissue
Abstract The first live birth to occur after ovarian-tissue transplantation between two genetically different sisters was reported in 2011. Since this is an acceptable practice with monozygotic twins, there is…
Chapter 37 – Predicting Ovarian Futures
Abstract The first live birth to occur after ovarian-tissue transplantation between two genetically different sisters was reported in 2011. Since this is an acceptable practice with monozygotic twins, there is…
Chapter 38 – Fertility Preservation
Abstract One of the consequences of cancer therapies, including radiation and chemotherapy, is gonadotoxicity. As effective treatments have rendered a number of malignancies curable, or have delivered long-term survival, post-treatment…
