81-BV-1a Blastocyst 4bb before vitrification, a cytoplasmic string extends from the inner cell mass to the trophectoderm (3 o’clock), some TE cells with vacuoles (at 12 o’clock position) 81-BV-1b Large,…

80-BV-1 Blastocyst 3ba before vitrification, flat inner cell mass, cohesive trophectoderm 80-BV-2 Blastocyst 5cb before vitrification, loose fragments close to inner cell mass and some necrotic cells, hatching at 4…

79-BV-1 Blastocyst 2bc before vitrification, loose trophectoderm, no cohesive cells 79-BV-2 Early blastocyst with thick ZP 79-AW-1 Blastocyst 2bc half contracted after warming, several cells are not included in blastocyst…

78-BV-1 Blastocyst 5ab before vitrification, compact inner cell mass in the background at 12 o’clock, hatching at 3 o’clock 78-BV-2a Blastocyst 4bb before artificial shrinkage 78-BV-2b Blastocyst 4bb after artificial…

75-BV-1 Blastocyst 4aa before vitrification, nice cohesive trophectoderm, inner cell mass compact, slightly visible in the background at 5 o’clock 75-BV-2 Blastocyst 4bc before vitrification, zona pellucida not fully thinned…

76-BV-1 Blastocyst 2bb in contraction phase before vitrification 76-BV-2 Bl 3cc before vitrification, poor trophectoderm, one necrotic cell at 12 o’clock position, thick zona pellucida

74-BV-1a Slightly contracting blastocyst, nice trophectoderm 74-BV-1b Large, compact inner cell mass 74-AW-1a After warming same appearance as before vitrification

72-BV-1 Fully expanded blastocyst with nice cohesive trophectoderm and a compact inner cell mass in the background, on the left a large vacuole formed by TE cells 72-BV-2 Expanded blastocyst…

73-BV-1 Before vitrification blastocyst hatching at 11 o’clock with flat inner cell mass 73-AW-1a Blastocyst fully re-expanded during the exposure to the sucrose solutions

71-BV-1 Blastocyst 4ba with flat, poorly defined inner cell mass before vitrification 71-BV-2 Blastocyst 4ca with nice cohesive trophectoderm and thinned zona pellucida 71-AW-1 Fully expanded blastocyst after warming