20-BV-1 Expanded blastocyst hatching from the embryonic pole

19-BV-1 Early blastocyst with blastocoel formation at the 2 o’clock position

18-BV-1 Transition from fully compacted stage to morula 18-AW-1a Immediate survival

17-BV-1 Expanded blastocyst with thick ZP 17-AW-1 Warmed blastocyst with extensive cytoplasmic granulation

16-BV-1 Expanded blastocyst starting to collapse in preparation for hatching 16-AW-1 Same blastocyst after warming, cytoplasm appears granulated

15-BV-1 Blastocyst showing beginning expansion 15-AW-1 Same blastocyst 2h after warming

14-BV-1 Day 5 morula of optimal quality 14-AW-1 Morula showing immediate survival

13-BV-1 Transition of early to full blastocyst stage 13-AW-1 Viable blastocyst 13-BT-1 Full blastocyst with reduced trophectoderm

12-BV-1 Expanded blastocyst (although zona is still thick) 12-AW-1 Warmed blastocyst showing excluded fragments

10-BV-1 Morula with excluded blastomere at 7 o’clock position 10-AW-1 Morula showing regression of cell–cell contacts