Temperature control in the IVF laboratory
(a) Heat conduction from an electric cooker hob plate; (b) Heat convection from an oil burner. (Quartec GmbH.) Box 6.2 Practical application of Newton’s Law of Cooling in forensic science…
Gases, air quality, and volatile organic carbons (VOCs)
(oil and grease)5 ppm w/w max.Phosphine***≤= 0.3 ppm v/vTotal volatile hydrocarbons (calculated as methane)50 ppm v/v max. of which 20 ppm v/v max non-methane hydrocarbons.Acetaldehyde0.2 ppm v/v max.Benzene0.02 ppm v/v…
Infections in IVF culture
Fig. 9.1 Microbial colonies accumulate in hand crevices and on tips of fingers, illustrated in darkened areas here. Box 9.1 Case report A patient had three routine ICSI procedures carried…
Troubleshooting ICSI procedures
Fig. 11.1 Micromanipulation station (Integra 3™, Research Instruments Ltd), with parts identified. (Image kindly supplied by Research Instruments Ltd, UK. www.research-instruments.com.) Location of the micromanipulation station Micromanipulation requires complete concentration,…
Troubleshooting a cryopreservation system
Table 10.1 Grade 5.0 liquid nitrogen specification (courtesy of Mr. Nick Brown, Air Products) Specification Typical analysis (by volume) Nitrogen > 99.999% > 99.999% Oxygen < 5 ppm < 2…
Managing risk in an IVF laboratory
(a) ICSI station. TS150 JRS scientific instruments/The Table Stable Ltd. (b) Benchtop incubator. Planer PLC. (Images kindly provided by Fertility Associates, Auckland, New Zealand.) If “safety” is theoretically interpreted as…
pH regulation in gamete and embryo culture systems
Fig. 5.1 Bavister’s model for regulation of intracellular pH via amino acid shuttling across the cell membrane. (Adapted with permission from: Bavister BD (1995). Hum Reprod Update 1(2): 91–148.) The…
The effect of light on embryos and embryo culture
Fig. 7.1 The spectrum of visible light; numbers on the left represent wavelength in nanometers. Ultraviolet light has wavelengths between approximately 310 nm and 100 nm, with photon energies from…
