8: A comparison of real time PCR and culture for the quantitative detection of selected vaginal microbiota




Objectives


To compare the frequency and concentrations of five bacteria commonly found in the vagina (Lactobacillus crispatus, L. iners, Gardnerella vaginalis, Atopobium vaginae, and Megasphera-Like bacteria phylotype I) by quantitative culture and real time PCR (qPCR).




Methods


Vaginal swabs for Nugent score, qPCR, and culture were collected from 61 asymptomatic healthy women aged 18-45 uninfected by C. trachomatis, N. gonorrhoeae, HIV, T. vaginalis and who did not meet the Amsel criteria for bacterial vaginosis (BV). Bacterial DNA from vaginal swabs for qPCR was extracted, combined with specific primers targeting each species and stained using SYBR green binding dye for detection (gene copies/swab). Bacteria isolated by culture [colony forming units/gram (CFU)] were identified using DNA based tests. Results were analyzed using Wilcoxon signed rank and McNemar’s tests.




Methods


Vaginal swabs for Nugent score, qPCR, and culture were collected from 61 asymptomatic healthy women aged 18-45 uninfected by C. trachomatis, N. gonorrhoeae, HIV, T. vaginalis and who did not meet the Amsel criteria for bacterial vaginosis (BV). Bacterial DNA from vaginal swabs for qPCR was extracted, combined with specific primers targeting each species and stained using SYBR green binding dye for detection (gene copies/swab). Bacteria isolated by culture [colony forming units/gram (CFU)] were identified using DNA based tests. Results were analyzed using Wilcoxon signed rank and McNemar’s tests.

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May 5, 2017 | Posted by in GYNECOLOGY | Comments Off on 8: A comparison of real time PCR and culture for the quantitative detection of selected vaginal microbiota
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