Methods
Vaginal swabs for Nugent score, qPCR, and culture were collected from 61 asymptomatic healthy women aged 18-45 uninfected by C. trachomatis, N. gonorrhoeae, HIV, T. vaginalis and who did not meet the Amsel criteria for bacterial vaginosis (BV). Bacterial DNA from vaginal swabs for qPCR was extracted, combined with specific primers targeting each species and stained using SYBR green binding dye for detection (gene copies/swab). Bacteria isolated by culture [colony forming units/gram (CFU)] were identified using DNA based tests. Results were analyzed using Wilcoxon signed rank and McNemar’s tests.
Methods
Vaginal swabs for Nugent score, qPCR, and culture were collected from 61 asymptomatic healthy women aged 18-45 uninfected by C. trachomatis, N. gonorrhoeae, HIV, T. vaginalis and who did not meet the Amsel criteria for bacterial vaginosis (BV). Bacterial DNA from vaginal swabs for qPCR was extracted, combined with specific primers targeting each species and stained using SYBR green binding dye for detection (gene copies/swab). Bacteria isolated by culture [colony forming units/gram (CFU)] were identified using DNA based tests. Results were analyzed using Wilcoxon signed rank and McNemar’s tests.
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