Methods
We enrolled women with and without BV in a study of the vaginal microbiota. Human genomic DNA was extracted from whole blood and used to genotype a panel of 14 single nucleotide polymorphisms (SNPs) in toll-like receptor-1 (TLR1), TLR2, TLR4, TLR5, TLR6, as well as regulators of TLR signaling, toll-interacting protein (TOLLIP), and CD180. In a cohort of 43 non-Hispanic, Caucasian participants, colonization with 15 bacterial species was assessed by quantitative PCR applied to DNA extracted from vaginal swabs. An independent cohort of 116 women without prevalent BV was followed for a period of approximately 90 days and assessed for BV by a study clinician at monthly intervals. DNA extracted from vaginal swabs collected at study enrollment was used to assess Lactobacillus colonization by quantitative PCR.
Methods
We enrolled women with and without BV in a study of the vaginal microbiota. Human genomic DNA was extracted from whole blood and used to genotype a panel of 14 single nucleotide polymorphisms (SNPs) in toll-like receptor-1 (TLR1), TLR2, TLR4, TLR5, TLR6, as well as regulators of TLR signaling, toll-interacting protein (TOLLIP), and CD180. In a cohort of 43 non-Hispanic, Caucasian participants, colonization with 15 bacterial species was assessed by quantitative PCR applied to DNA extracted from vaginal swabs. An independent cohort of 116 women without prevalent BV was followed for a period of approximately 90 days and assessed for BV by a study clinician at monthly intervals. DNA extracted from vaginal swabs collected at study enrollment was used to assess Lactobacillus colonization by quantitative PCR.
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