Objectives
Vitamin D3 insufficiency during pregnancy is associated with an increased risk of infection associated pregnancy complications including preterm labor. Vitamin D3 supplementation reduced pregnancy complications. However the mechanism of how the vitamin D3 supplementation leads to prevention of infection associated preterm labor and other inflammatory pregnancy complications is not clear. Here we attempt to identify vitamin D3 effect on resolution of LPS induced pro-inflammatory signaling in primary monocytes derived from pregnant woman.
Methods
We recruited healthy pregnant subjects (23-35 weeks of gestation) for maternal blood collection. We established an ex vivo experimental system model with primary monocytic cells isolated from maternal blood. Primary monocytes supplemented with vitamin D3 or vehicles for control, were exposed to E. coli LPS in a time dependent manner. Quantitative PCR (RT-PCR), quantitative immunofluorescent and 3-D confocal microscopy were used to quantify molecular changes in mRNA pools and surface protein expression normalized to 18s rRNA and cellular DNA content, respectively.
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