2: Comparison of three methods for diagnosis of trichomonas vaginalis infections




Objectives


According to the 2015 STD Treatment Guidelines released by the Centers for Disease Control and Prevention, Trichomoniasis is the most prevalent nonviral STI in the US. Although traditionally diagnosed by wet mount microscopy, many infections go undetected due to the low sensitivity of this method (51-65%). An evaluation of an antigen based test and a PCR assay was performed to determine the best diagnostic method.




Methods


Diagnosis of Trichomonas infection by wet mount microscopy is based on observation of a motile organism. The OSOM Trichomonas rapid test uses immunochromatographic technology to detect antigens from vaginal swabs collected from symptomatic patients or those with suspected exposure. The Cepheid Xpert TV assay uses PCR to detect T. vaginalis DNA, and is intended to diagnose infection in symptomatic or asymptomatic females using either first-catch urine or vaginal/endocervical swabs. Ninety-nine specimens submitted for wet mount examination were tested using microscopy, the OSOM Trichomonas kit, and Xpert TV PCR testing.




Methods


Diagnosis of Trichomonas infection by wet mount microscopy is based on observation of a motile organism. The OSOM Trichomonas rapid test uses immunochromatographic technology to detect antigens from vaginal swabs collected from symptomatic patients or those with suspected exposure. The Cepheid Xpert TV assay uses PCR to detect T. vaginalis DNA, and is intended to diagnose infection in symptomatic or asymptomatic females using either first-catch urine or vaginal/endocervical swabs. Ninety-nine specimens submitted for wet mount examination were tested using microscopy, the OSOM Trichomonas kit, and Xpert TV PCR testing.

Only gold members can continue reading. Log In or Register to continue

Stay updated, free articles. Join our Telegram channel

May 2, 2017 | Posted by in GYNECOLOGY | Comments Off on 2: Comparison of three methods for diagnosis of trichomonas vaginalis infections

Full access? Get Clinical Tree

Get Clinical Tree app for offline access