Objectives
Cervicitis is an inflammatory condition of the cervix associated with upper genital tract infection and reproductive complications. Although cervicitis can be caused by several known pathogens, the etiology frequently remains obscure. Here we investigate vaginal bacteria associated with bacterial vaginosis as potential causes of cervicitis.
Methods
Relationships of cervical and vaginal bacteria with cervicitis were examined in a retrospective case control study of women attending a Seattle STD clinic. Individual bacterial species were detected using two molecular methods: quantitative PCR (qPCR) and broad range 16S rRNA gene PCR with pyrosequencing. Associations of species presence and abundance with cervicitis were determined using exact logistic regression for the qPCR data, and lasso regression with binomial response (accounting for multiple comparisons) for the pyrosequencing data. The primary finding from this initial study was evaluated using qPCR in a validation cohort of Kenyan women.
Methods
Relationships of cervical and vaginal bacteria with cervicitis were examined in a retrospective case control study of women attending a Seattle STD clinic. Individual bacterial species were detected using two molecular methods: quantitative PCR (qPCR) and broad range 16S rRNA gene PCR with pyrosequencing. Associations of species presence and abundance with cervicitis were determined using exact logistic regression for the qPCR data, and lasso regression with binomial response (accounting for multiple comparisons) for the pyrosequencing data. The primary finding from this initial study was evaluated using qPCR in a validation cohort of Kenyan women.
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