Methods
This study was conducted in two phases. In phase 1, a feasibility trial was conducted, in which 20 healthy pregnant women were enrolled between 14-28 weeks’ gestation and two maternal rectal swabs were obtained. In phase 2, 31 pregnant women were enrolled at < 20 weeks’ gestation and had a second study visit at 36-39 weeks gestation. Maternal rectal swabs were collected at both study visits. In phase 1, we described microbiome diversity. In phase 2, we compared microbiome diversity between two collection time points. Bacterial DNA was extracted from swabs, followed by 16s rRNA gene-based PCR and multiplex high-throughput sequencing (Illumina MiSeQ) and analyzed using Qiime, PRIMER VI (Devon, UK). A total of six phyla and 70 genera were identified. We used cluster analysis to determine whether bacterial profiles by phylum and genus differed by gestational age and longitudinally through pregnancy. We also examined if there were differences in diversity by maternal body mass index (BMI) race and gestational weight gain (GWG) using Spearman’s correlation.
Methods
This study was conducted in two phases. In phase 1, a feasibility trial was conducted, in which 20 healthy pregnant women were enrolled between 14-28 weeks’ gestation and two maternal rectal swabs were obtained. In phase 2, 31 pregnant women were enrolled at < 20 weeks’ gestation and had a second study visit at 36-39 weeks gestation. Maternal rectal swabs were collected at both study visits. In phase 1, we described microbiome diversity. In phase 2, we compared microbiome diversity between two collection time points. Bacterial DNA was extracted from swabs, followed by 16s rRNA gene-based PCR and multiplex high-throughput sequencing (Illumina MiSeQ) and analyzed using Qiime, PRIMER VI (Devon, UK). A total of six phyla and 70 genera were identified. We used cluster analysis to determine whether bacterial profiles by phylum and genus differed by gestational age and longitudinally through pregnancy. We also examined if there were differences in diversity by maternal body mass index (BMI) race and gestational weight gain (GWG) using Spearman’s correlation.
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