Methods
Twenty chronically catheterized pregnant monkeys (Macaca nemestrina) at 118-125 days gestation (term=172 days) received choriodecidual inoculation of either Group B Streptococcus (N=13), E. coli (n=1), lipopolysaccharide (N=1) or saline (n=5). Amniotic fluid (AF), maternal serum, and fetal serum samples were taken at regular time intervals after inoculation and cytokine levels were determined by Luminex assay. Cesarean section was performed at the time of preterm labor or within 4-7 days of inoculation depending on the protocol. Histopathology of the placenta was analyzed by Redline criteria (chorioamnion, umbilical cord, villi, decidua) or assigned a fetal lung score indicative of damage by pathologists blinded to experimental group. Correlation of data was performed using Spearman’s rank correlation and Wilcoxon rank sum analysis.
Methods
Twenty chronically catheterized pregnant monkeys (Macaca nemestrina) at 118-125 days gestation (term=172 days) received choriodecidual inoculation of either Group B Streptococcus (N=13), E. coli (n=1), lipopolysaccharide (N=1) or saline (n=5). Amniotic fluid (AF), maternal serum, and fetal serum samples were taken at regular time intervals after inoculation and cytokine levels were determined by Luminex assay. Cesarean section was performed at the time of preterm labor or within 4-7 days of inoculation depending on the protocol. Histopathology of the placenta was analyzed by Redline criteria (chorioamnion, umbilical cord, villi, decidua) or assigned a fetal lung score indicative of damage by pathologists blinded to experimental group. Correlation of data was performed using Spearman’s rank correlation and Wilcoxon rank sum analysis.
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