9: Utilization of molecular methods to evaluate vaginal microbiota in a longitudinal study of recurrent bacterial vaginosis




Objectives


Our goal was to evaluate the impact of antimicrobial treatment on the presence and quantity of selected bacterial species in a recurrent bacterial vaginosis (BV) population.




Methods


Eighty-four subjects were evaluated by clinical signs (i.e. Amsel’s criteria) and Gram-stain of vaginal smears (i.e. Nugent scoring). In addition, a panel of quantitative real-time polymerase chain reaction (qPCR) assays was used to evaluate the vaginal flora. Subjects were evaluated at the index episode, 7-10 days later (post-treatment) and 40-45 days later.




Methods


Eighty-four subjects were evaluated by clinical signs (i.e. Amsel’s criteria) and Gram-stain of vaginal smears (i.e. Nugent scoring). In addition, a panel of quantitative real-time polymerase chain reaction (qPCR) assays was used to evaluate the vaginal flora. Subjects were evaluated at the index episode, 7-10 days later (post-treatment) and 40-45 days later.

Only gold members can continue reading. Log In or Register to continue

Related posts:

  1. Predictive accuracy of changes in transvaginal sonographic cervical length over time for preterm birth: a systematic review and metaanalysis
  2. 2: Longitudinal study evaluating the impact of hormonal contraception on vaginal microflora patterns
  3. 9: The prevalence of chlamydia in adolescents at presentation to colposcopy: do new cervical cancer screening guidelines eliminate an STI screening opportunity?
  4. 7: The incidence of septicemia at delivery and six week readmission in three large states

Stay updated, free articles. Join our Telegram channel
Tags:
May 5, 2017 | Posted by in GYNECOLOGY | Comments Off on 9: Utilization of molecular methods to evaluate vaginal microbiota in a longitudinal study of recurrent bacterial vaginosis

Full access? Get Clinical Tree
Get Clinical Tree app for offline access