Objectives
The lack of an efficacious vaccine warrants further understanding of the basic biology of Chlamydia trachomatis infection, the leading STD in the United States and cause of infections blindness worldwide. Chlamydia spp. utilize effector proteins to facilitate invasion and infection. Here, we aim to elucidate the function of one such effector protein, CT694.
Methods
HeLa and wild-type or Ahnak -/- MEF cell lines were infected with purified infectious C. trachomatis. A variety of molecular and microbiology techniques were employed, including cloning and RT-PCR, transfection and transduction, co-immunoprecipitation, western blotting, actin binding protein assays, cell culture, and attachment and invasion assays. Visualization was obtained by immunofluorescence, confocal, and transmission electron microscopy. Statistical analyses were performed with Prism.
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