29: Prevalence of cytomegalovirus infection in placentas and umbilical cords with inflammation from unexplained small for gestational age neonates: A pilot study




Objectives


The purpose of this study was to investigate the prevalence of cytomegalovirus (CMV) infection by PCR in placentas and umbilical cords from infants born with unexplained small for gestational age (SGA) when there is evidence of inflammation in the placenta.




Methods


A cohort of SGA neonates was identified from the electronic medical record (EMR) of a suburban tertiary care center. SGA infants were identified according to sex-specific weight determinants as previously reported (Kramer et al., 2001). The corresponding gestational age at delivery, birth weight, maternal and neonatal laboratory results for infectious diseases (CMV, toxoplasmosis, and parvovirus), maternal history, medication and drug use were collected from the EMR. Pregnancies complicated by multiple gestation, fetal anomalies, maternal hypertension/preeclampsia or diabetes, abnormal cord insertion, and maternal tobacco use were excluded. PCR for CMV DNA was performed using an in-house real-time PCR assay performed on a Light Cycler utilizing FRET probes for fluorescent detection. DNA was prepared from deparaffinized sections of formalin fixed, paraffin-embedded placental and umbilical cord tissue, and was evaluated for successful amplification of a 200 bp fragment of human DNA prior to CMV PCR.

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May 2, 2017 | Posted by in GYNECOLOGY | Comments Off on 29: Prevalence of cytomegalovirus infection in placentas and umbilical cords with inflammation from unexplained small for gestational age neonates: A pilot study

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