Predictors of response to 17-alpha hydroxyprogesterone caproate for prevention of recurrent spontaneous preterm birth




Materials and Methods


This is a planned analysis of a multicenter, prospectively collected longitudinal study of women who were enrolled in the Eunice Kennedy Shriver National Institute for Child Health and Human Development Genomic and Proteomic Network for Preterm Birth Research. Briefly, women with a history of a documented singleton spontaneous PTB between 20 weeks 0 days gestation and 36 weeks 6 days gestation were recruited across 8 clinical sites from November 2007 through January 2011 and were followed prospectively. All women had an initial study visit between 10 weeks 0 days and 18 weeks 6 days gestation. Gestational age was determined by a combination of last menstrual period (if available) and ultrasound scanning. Because all patients enrolled at <19 weeks gestation, the gestational age was assigned by ultrasound scanning if the ultrasound dating varied by ±7 days from the last menstrual period.


Clinical and demographic data were collected by trained research nurses. Data were collected at 2 additional prespecified return visits (visit 2: 18 weeks 0 days–23 weeks 6 days gestation; visit 3: 28 weeks 0 days–31 weeks 6 days gestation) and at delivery. Additional data were collected if an enrolled participant was admitted to the hospital with a diagnosis of preterm labor or PPROM. Research nurses conducted in-person interviews with participants during study visits and abstracted additional clinical and demographic data from medical records. Data that were collected included demographics; medical, social, family, and obstetric histories; and obstetric course and complications during the current pregnancy (which included intrapartum course, mode of delivery, and neonatal outcomes). Obstetric management was per each woman’s primary obstetric provider. This study was approved by the Institutional Review Board at each center; written informed consent was obtained from all participants.


For the purposes of this analysis, we included only women who received at least 1 dose of 17OHP-C during pregnancy for the prevention of recurrent PTB. We classified women as a 17OHP-C responder or nonresponder by calculating the difference in the gestational age at delivery between the 17OHP-C–treated pregnancy and her earliest SPTB, as we have described previously. Specifically, the difference between the earliest delivery gestational age because of SPTB and the delivery gestational age with 17OHP-C was calculated and termed the “17OHP-C effect.” Women with a 17OHP-C effect of ≥3 weeks (ie, the individual’s pregnancy or pregnancies that were treated with 17OHP-C delivered at least 3 weeks later compared with the gestational age of the earliest SPTB without 17OHP-C treatment) were considered 17OHP-C responders. Women with a negative overall 17OHP-C effect and those with an overall 17OHP-C effect of <3 weeks were classified as nonresponders. Women with a 17OHP-C effect of <3 weeks but who delivered at term (eg, if the earliest previous SPTB was at 35 weeks gestation and if the patient delivered during the 17OHP-C treated pregnancy at 37 weeks gestation) were considered “equivocal” 17OHP-C responders and were excluded from analysis.


Univariable analyses were conducted by using chi-square, t -test, and analysis of variance, as appropriate. Multivariable analysis was performed by the use of stepwise backward elimination logistic regression, and factors with a probability value of <.20 remained in the final models. All statistical analyses were performed with STATA software (version 13.1; Stata Corporation, College Station, TX). A probability value of <.05 was considered statistically significant.




Results


From the original cohort of 517 women, 166 women (33.0%) received at least 1 dose of 17OHP-C and met inclusion criteria ( Figure 1 ). Eleven women had a 17OHP-C effect at <3 weeks yet delivered at term because their earliest previous PTB was >34 weeks gestation; these women were considered equivocal responders and were excluded. Of the remaining women, 118 of 155 women (76%) delivered at least 3 weeks later compared with the delivery gestational age of their previous earliest PTB and were considered 17OHP-C responders; 37 women (24%) had a 17OHP-C effect at <3 weeks and were considered nonresponders. The 118 responders delivered later on average (37.7 weeks gestation) than the 37 nonresponders (33.5 weeks gestation; P < .001). Although 32% of responders (38/118 women) had a recurrent SPTB, they were considered responders because they delivered ≥3 weeks later compared with their earliest SPTB. The gestational age of the earliest previous PTB and the delivery gestational age of the current studied pregnancy with 17OHP-C are plotted for each subject in Figure 2 .




Figure 1


Study enrollment

Earliest previous preterm birth was 35-36 weeks gestation, which was delivered during the early term period (37-38 weeks) gestation.

17 P , 17-alpha hydroxyprogesterone caproate; GPN , Genomics and Proteomics Network for Preterm Birth.

Manuck et al. Clinical response to 17OHP-C for preterm birth prevention. Am J Obstet Gynecol 2016 .



Figure 2


Gestational and delivery age

The gestational age of the earliest previous preterm birth and the delivery gestational age of the current studied pregnancy with 17-alpha hydroxyprogesterone caproate are plotted for each subject. The red bars indicate that the delivery gestational age was earlier with 17-alpha hydroxyprogesterone caproate compared with the gestational age of that subject’s earliest previous preterm birth. The green bars indicate that the delivery gestational age was later with 17-alpha hydroxyprogesterone caproate compared with the gestational age of that subject’s earliest previous preterm birth.

17 P , 17-alpha hydroxyprogesterone caproate.

Manuck et al. Clinical response to 17OHP-C for preterm birth prevention. Am J Obstet Gynecol 2016 .


Demographic characteristics were similar between responders and nonresponders ( Table 1 ). Characteristics of the previous pregnancy were also similar, with some notable differences. Although the gestational age of their earliest previous SPTB was later and a similar proportion had a history of a term delivery, nonresponders were more likely to have delivered their penultimate pregnancy preterm ( Table 1 ). They were also more likely to have either a mother or sister with a history of a PTB at <37 weeks gestation ( Table 1 ). Women who did not respond to 17OHP-C were more likely to have ≥1 episodes of vaginal bleeding during pregnancy or to be diagnosed with a clinical abruption ( Table 2 ).



Table 1

Demographic factors and pregnancy history by 17-alpha hydroxyprogesterone caproate responder status










































































































Variable 17-Alpha hydroxyprogesterone caproate P value
Nonresponder (n = 37) Responder (n = 118)
Maternal age, y a 27.0 ± 4.9 27.7 ± 5.1 .470
Maternal race, n (%) .803
White 24 (64.9) 72 (61.0)
African-American 12 (32.4) 39 (33.1)
Other 1 (2.7) 7 (5.9)
Maternal Hispanic ethnicity, n (%) 3 (8.1) 18 (15.3) .268
High school education or higher, n (%) 29 (78.4) 89 (75.4) .713
Insurance status, n (%) .070
Public 16 (43.2) 62 (52.5)
Private 20 (54.1) 46 (39.0)
Self-pay 1 (2.7) 10 (8.5)
Gestational age of earliest previous spontaneous preterm birth, wk a 33.5 ± 2.4 29.2 ± 5.0 < .001
Previous spontaneous preterm births, n a 1.5 ± 0.7 1.6 ± 1.0 .531
>1 Previous spontaneous preterm birth, n (%) 19 (51.4) 42 (35.6) .087
History of a spontaneous preterm birth in the penultimate pregnancy reaching at least 20 weeks gestation, n (%) 35 (94.6) 95 (80.5) .042
≥1 Previous term birth(s), n (%) 14 (37.8) 39 (33.1) .590
History of abruption, n (%) 3 (8.1) 2 (1.7) .054
History of preterm premature rupture of membranes in ≥1 previous pregnancies (20-37 weeks gestation), n (%) 5 (13.5) 13 (11.0) .679
Sister and/or mother with preterm birth, n (%) 19 (51.4) 38 (32.2) .035

Manuck et al. Clinical response to 17OHP-C for preterm birth prevention. Am J Obstet Gynecol 2016 .

a Data are given as mean ± SD.



Table 2

Current pregnancy factors by 17-alpha hydroxyprogesterone caproate responder status












































































Variable 17-Alpha hydroxyprogesterone caproate P value
Nonresponder (n = 37) Responder (n = 118)
Prepregnancy body mass index, kg/m 2 a 25.9 ± 6.3 27.5 ± 7.3 .251
Smoked during pregnancy, n (%) 3 (8.1) 8 (6.7) .784
≥1 Episode(s) of vaginal bleeding or diagnosis of clinical abruption, n (%) 7 (18.9) 9 (7.6) .049
Bacterial vaginosis, n (%) 4 (10.8) 17 (14.4) .577
Gonorrhea and/or chlamydia, n (%) 2 (5.4) 10 (8.5) .542
Short cervix <2.50 cm, n (%) b 3 (8.1) 9 (7.6) .924
Patient reported regular contractions at time of routine outpatient follow-up appointment, n (%) 17 (46.0) 35 (30.2) .078
Received any tocolysis, n (%) 16 (43.2) 34 (28.8) .101
Received indomethacin 4 (10.8) 7 (5.9) .313
Received magnesium sulfate 3 (8.1) 13 (11.0) .612
Received nifedipine 14 (37.8) 21 (17.8) .011
Received antenatal corticosteroids, n (%) 19 (51.4) 26 (22.0) .001
Male fetus, n (%) 23 (62.2) 58 (49.2) .167

Manuck et al. Clinical response to 17OHP-C for preterm birth prevention. Am J Obstet Gynecol 2016 .

a Data are given as mean ± SD


b Among 95 women with transvaginal cervical length assessment.



Of note, women with a prophylactic cervical cerclage were excluded from enrollment in the main study. However, 6 women underwent cerclage placement because of cervical shortening or cervical dilation, which was identified by either physical or ultrasound examination; all 6 women were classified as 17OHP-C responders. These 6 women delivered at a mean of 35.5 weeks gestation (range, 28.9-39.6 weeks gestation). No study participant was noted to also be taking vaginal progesterone in the second trimester, although 7 women (3 nonresponders and 4 responders; P = .196) were prescribed first-trimester supplementation at the discretion of their primary obstetric care provider.


In the regression model, each additional week of gestation of the earliest previous SPTB (odds ratio, 0.68; 95% confidence interval, 0.56–0.82; P < .001), vaginal bleeding/abruption in the current pregnancy (odds ratio, 0.24; 95% confidence interval, 0.06–0.88; P = .031), and first-degree family history of SPTB (odds ratio, 0.37; 95% confidence interval, 0.15–0.88; P = .024) were associated with response to 17OHP-C ( Table 3 ). Other factors that were considered in the initial model but were removed from final models because of a probability value of >.20 included a history of abruption in a previous pregnancy, insurance status, and a male fetus.



Table 3

Multivariable logistic regression model: factors that are associated with 17-alpha hydroxyprogesterone caproate response in the overall cohort of 155 women





























Characteristic Odds ratio 95% Confidence interval P value
Gestational age of earliest previous spontaneous preterm birth, wk 0.68 0.56–0.82 < .001
First-degree family history of spontaneous preterm birth 0.37 0.15–0.88 .024
Vaginal bleeding or abruption in current pregnancy 0.24 0.06–0.88 .031
Immediately antecedent pregnancy delivered preterm <37 weeks gestation 0.21 0.03–1.19 .079

Manuck et al. Clinical response to 17OHP-C for preterm birth prevention. Am J Obstet Gynecol 2016 .


Because women with a penultimate preterm pregnancy were more likely to be 17OHP-C nonresponders, we performed an additional limited analysis examining only the 130 women whose penultimate pregnancy was preterm. With the use of the definition of 17OHP-C responder, 95 of 130 women (73.1%) were considered 17OHP-C responders, and 35 of 130 women (26.9%) were nonresponders. The antecedent delivery gestational age remained earlier among the group classified as responders (30.6 ± 4.5 vs 33.9 ± 2.4 weeks gestation; P < .001); other baseline and demographic characteristics were also similar between groups (data not shown). In regression models, the results were similar to those in the main cohort ( Table 4 ). To further assess the impact of the delivery gestational age of the penultimate pregnancy, we used the gestational age of the penultimate pregnancy (instead of the gestational age of the earliest PTB) to calculate the 17OHP-C effect, as described earlier. This classified 86 of 130 women (66.2%) as 17OHP-C responders, 41 of 130 (31.5%) as 17OHP-C nonresponders, and 3 of 130 (2.3%) as equivocal 17OHP-C responders. Again, the gestational age of the earliest previous PTB was earlier among 17OHP-C responders (29.6 ± 2.9 vs 32.5 ± 3.8 weeks gestation; P = .001). Overall regression models remained similar with the use of this modified definition of 17OHP-C responder, with later gestational age of the earliest previous PTB and positive family history both reducing the odds of 17OHP-C response, although the presence of vaginal bleeding or abruption did not remain associated with 17OHP-C response in this final model.



Table 4

Multivariable logistic regression model: factors that are associated with 17-alpha hydroxyprogesterone caproate response among the 130 women with a penultimate preterm birth





























Characteristic Odds ratio 95% Confidence interval P value
Gestational age of earliest previous spontaneous preterm birth, wk 0.68 0.56–0.83 < .001
First-degree family history of spontaneous preterm birth 0.49 0.20–1.23 .130
Vaginal bleeding or abruption in current pregnancy 0.39 0.09–1.57 .183
History of abruption in ≥1 previous pregnancies 0.11 0.01–1.46 .094

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May 4, 2017 | Posted by in GYNECOLOGY | Comments Off on Predictors of response to 17-alpha hydroxyprogesterone caproate for prevention of recurrent spontaneous preterm birth

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