• 
  

  • Central nervous system (CNS) infection (viral, fungal, or bacterial) or malignancy.

  
  

  • Intracranial pressure or pseudotumor.

  
  

  • Metabolic studies.

  
  

  • Aminoacidopathies.

  
  

  • Neurotransmitter disorders.

  
  
  
  
  • 
    

    • Undiagnosed movement disorders.

    
    

    • Undiagnosed infantile or pediatric epilepsy.

  
  

  • Demyelinating disease (eg, multiple sclerosis).

Absolute

• 
  

  • CNS herniation.

  
  

  • Unilateral mass lesion with edema or mass effect.

Relative

• 
  

  • Suspected focal mass lesion.

• 
  

  • Spinal needle: 0.5 inch for neonate, 22 gauge.

  
  

  • Manometer.

  
  

  • Sterile collection tubes (sufficient number for studies).

  
  

  • 3-way stopcock.

  
  

  • Flexible tubing.

• 
  

  • Herniation (extremely rare) is associated with focal structural lesions causing increased intracranial pressure.

  
  

  • Infection (extremely rare).

  
  

  • Headache (rare).

  
  

  • Back pain.

• 
  

  • Placing the patient with the sacral plane vertical is key.

  
  

  • The head of the patient should be on your nondominant side. (Left-handed physicians should place the patient in the left lateral decubitus position.)

  
  

  • Use your nondominant thumb to palpate the spinous process of L4 and put your index finger on the iliac crest.

  
  

  • Use your dominant hand to manipulate the needle.

  
  

  • If positioning is felt to be correct, try rotating the needle 90 degrees.

  
  

  • If cerebrospinal fluid (CSF) flows slowly, be patient.

• 
  

  • Sterile technique.

  
  

  • Povidone-iodine preparation.

  
  

  • Sterile drape with fenestration over midlumbar spine.

  
  

  • Sedation, if needed.

  
  

  • Connect 3-way stopcock to flexible tubing and manometer at 90 degrees from each other.

  
  

  • Free end of tubing will connect to hub of needle.